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Western Blot analysis of various samples using NOX4 Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of various samples using NOX4 Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of various samples using NOX4 Polyclonal Antibody at dilution of 1:1000.

NOX4 Polyclonal Antibody

E-AB-70215
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NOX4 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NADPH oxidase 4/NOX4 using anti-NADPH oxidase 4/NOX4 antibody (PA1929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human HK-2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SW579 whole cell lysates, Lane 7: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4/NOX4 antigen affinity purified polyclonal antibody (Catalog # PA1929) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NADPH OXIDASE 4/NOX4 at approximately 67KD. The expected band size for NADPH OXIDASE 4/NOX4 is at 67KD.
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