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WB analysis of mouse liver lysate (35ug) using NQO2 antibody at a dilution of 1:500.
WB analysis of mouse liver lysate (35ug) using NQO2 antibody at a dilution of 1:500.
WB analysis of mouse liver lysate (35ug) using NQO2 antibody at a dilution of 1:500.

NQO2 antibody [AT1E3]

GTX53753
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNQO2
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Overview

  • Supplier
    GeneTex
  • Product Name
    NQO2 antibody [AT1E3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:500
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT1E3
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4835
  • Target name
    NQO2
  • Target description
    N-ribosyldihydronicotinamide:quinone reductase 2
  • Target synonyms
    DHQV; DIA6; NAD(P)H dehydrogenase, quinone 2; NAD(P)H menadione oxidoreductase-1, dioxin-inducible-2; NAD(P)H quinone dehydrogenase 2; NMOR2; NRH:quinone oxidoreductase 2; QR2; quinone reductase 2; ribosyldihydronicotinamide dehydrogenase; ribosyldihydronicotinamide dehydrogenase [quinone]
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16083
  • Protein Name
    Ribosyldihydronicotinamide dehydrogenase [quinone]
  • Scientific Description
    This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NQO2 using anti-NQO2 antibody (A03112-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO2 antigen affinity purified polyclonal antibody (Catalog # A03112-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO2 at approximately 26 kDa. The expected band size for NQO2 is at 26 kDa.
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