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IHC-P analysis of human skin, melanoma tissue using GTX71803 NR1D2 antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human skin, melanoma tissue using GTX71803 NR1D2 antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human skin, melanoma tissue using GTX71803 NR1D2 antibody. Antigen retrieval : Heat-induced antigen retrieval

NR1D2 antibody

GTX71803
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mammals, Monkey, Rabbit
TargetNR1D2
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Overview

  • Supplier
    GeneTex
  • Product Name
    NR1D2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 4 - 7 microg/ml. IHC-P: 4 - 7 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9975
  • Target name
    NR1D2
  • Target description
    nuclear receptor subfamily 1 group D member 2
  • Target synonyms
    BD73, EAR-1R, REVERBB, REVERBbeta, RVR, nuclear receptor subfamily 1 group D member 2, V-erbA-related protein 1-related, orphan nuclear hormone receptor BD73, rev-erb alpha-related receptor, rev-erb-beta, rev-erba-alpha-related receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14995
  • Protein Name
    Nuclear receptor subfamily 1 group D member 2
  • Scientific Description
    This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]
  • Reactivity
    Bovine, Canine, Equine, Human, Mammals, Monkey, Rabbit
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NR1D2 using anti-NR1D2 antibody (A04958-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1D2 antigen affinity purified polyclonal antibody (Catalog # A04958-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1D2 at approximately 68 kDa. The expected band size for NR1D2 is at 65 kDa.
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