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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA016048ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA016048ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA016048ESR1HU at dilution of 1:100

NR1I2 Antibody

CSB-PA016048ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNR1I2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NR1I2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8856
  • Target name
    NR1I2
  • Target description
    nuclear receptor subfamily 1 group I member 2
  • Target synonyms
    BXR; nuclear receptor subfamily 1 group I member 2; ONR1; orphan nuclear receptor PAR1; orphan nuclear receptor PXR; PAR; PAR1; PAR2; PARq; pregnane X nuclear receptor variant 2; pregnane X receptor; PRR; PXR; SAR; steroid and xenobiotic receptor; SXR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75469
  • Protein Name
    Nuclear receptor subfamily 1 group I member 2
  • Scientific Description
    Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NR1I2 using anti-NR1I2 antibody (A01133-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1I2 antigen affinity purified polyclonal antibody (Catalog # A01133-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1I2 at approximately 50KD. The expected band size for NR1I2 is at 50KD.
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