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Paraformaldehyde-fixed, paraffin embedded Mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PXR Polyclonal Antibody, Unconjugated (bs-2334R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PXR Polyclonal Antibody, Unconjugated (bs-2334R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PXR Polyclonal Antibody, Unconjugated (bs-2334R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

PXR Polyclonal Antibody

BS-2334R
Bioss
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Equine, Human, Mouse, Porcine, Rat
TargetNR1I2
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Overview

  • Supplier
    Bioss
  • Product Name
    PXR Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8856
  • Target name
    NR1I2
  • Target description
    nuclear receptor subfamily 1 group I member 2
  • Target synonyms
    BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR, nuclear receptor subfamily 1 group I member 2, orphan nuclear receptor PAR1, orphan nuclear receptor PXR, pregnane X receptor, steroid and xenobiotic receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75469
  • Protein Name
    Nuclear receptor subfamily 1 group I member 2
  • Reactivity
    Bovine, Equine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Mohandas S, Vairappan B. Pregnane X receptor activation by its natural ligand Ginkgolide-A improves tight junction proteins expression and attenuates bacterial translocation in cirrhosis. Chem Biol Interact. 2020,315:108891. doi: 10.1016/j.cbi.2019.108891
    Read this paper
  • Sun B, Gao J, Shi W, et al. The interleukin-17 G-197A polymorphism is associated with cyclosporine metabolism and transplant rejection in liver transplant recipients. Pharmacogenomics. 2019,20(6):447-456. doi: 10.2217/pgs-2018-0198
    Read this paper
  • Zhou X, Li X, Wang X, et al. Cecropin B Represses CYP3A29 Expression through Activation of the TLR2/4-NF-κB/PXR Signaling Pathway. Sci Rep. 2016,6:27876. doi: 10.1038/srep27876
    Read this paper

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Figure 1. Western blot analysis of NR1I2 using anti-NR1I2 antibody (A01133-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1I2 antigen affinity purified polyclonal antibody (Catalog # A01133-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1I2 at approximately 50KD. The expected band size for NR1I2 is at 50KD.
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