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Western Blot analysis of 293 cells using EAR2 Polyclonal Antibody
Western Blot analysis of 293 cells using EAR2 Polyclonal Antibody
Western Blot analysis of 293 cells using EAR2 Polyclonal Antibody

NR2F6 Antibody

CSB-PA002242
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNR2F6
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Overview

  • Supplier
    Cusabio
  • Product Name
    NR2F6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2063
  • Target name
    NR2F6
  • Target description
    nuclear receptor subfamily 2 group F member 6
  • Target synonyms
    EAR2; EAR-2; ERBAL2; ERBA-related gene-2; nuclear receptor subfamily 2 group F member 6; nuclear receptor V-erbA-related; v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2; V-erbA-related protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10588
  • Protein Name
    Nuclear receptor subfamily 2 group F member 6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NR2F6 using anti-NR2F6 antibody (PB10081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR2F6 antigen affinity purified polyclonal antibody (Catalog # PB10081) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR2F6 at approximately 43 kDa. The expected band size for NR2F6 is at 43 kDa.
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