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ProductsBack/NR5A2 / LRH-1 Antibody

NR5A2 / LRH-1 Antibody

LS-C748724
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNR5A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NR5A2 / LRH-1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 42kDa/53kDa/56kDa/61kDa, while the observed MW by Western blot was 61kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 42kDa/53kDa/56kDa/61kDa, while the observed MW by Western blot was 61kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2494
  • Target name
    NR5A2
  • Target description
    nuclear receptor subfamily 5 group A member 2
  • Target synonyms
    B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2, nuclear receptor subfamily 5 group A member 2, CYP7A promoter-binding factor, b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus, fetoprotein-alpha 1 (AFP) transcription factor, hepatocytic transcription factor hB1F-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue, Mouse brain tissue All lanes: NR5A2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62, 57, 43, 54 kDa Observed band size: 62, 54 kDa
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Figure 1. Western blot analysis of NR5A2 using anti-NR5A2 antibody (A01332-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR5A2 antigen affinity purified polyclonal antibody (Catalog # A01332-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR5A2 at approximately 70 kDa. The expected band size for NR5A2 is at 61 kDa.
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