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ProductsBack/NSE antibody [5A4]

NSE antibody [5A4]

GTX10169
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetENO2
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Overview

  • Supplier
    GeneTex
  • Product Name
    NSE antibody [5A4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5A4
  • Conjugate
    Unconjugated
  • Gene ID2026
  • Target name
    ENO2
  • Target description
    enolase 2
  • Target synonyms
    HEL-S-279, NSE, gamma-enolase, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolyase, enolase 2 (gamma, neuronal), epididymis secretory protein Li 279, neural enolase, neuron specific gamma enolase, neuron-specific enolase, neuronal enriched enolase, neurone-specific enolase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP09104
  • Protein Name
    Gamma-enolase
  • Scientific Description
    This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Masi I, Caprara V, Spadaro F, et al. Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK. Cell Rep. 2021,34(9):108800. doi: 10.1016/j.celrep.2021.108800
    Read this paper
  • Lee YK, Kwon SM, Lee EB, et al. Mitochondrial Respiratory Defect Enhances Hepatoma Cell Invasiveness via STAT3/NFE2L1/STX12 Axis. Cancers (Basel). 2020,12(9). doi: 10.3390/cancers12092632
    Read this paper
  • Fischer N, Brandner J, Fuchs F, et al. Detection of Merkel cell polyomavirus (MCPyV) in Merkel cell carcinoma cell lines: cell morphology and growth phenotype do not reflect presence of the virus. Int J Cancer. 2010,126(9):2133-42. doi: 10.1002/ijc.24877
    Read this paper

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Figure 1. Western blot analysis of NSE using anti-NSE antibody (A02930). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysate, Lane 2: human U20S whole cell lysate, Lane 3: human A431 whole cell lysate, Lane 4: human HepG2 whole cell lysate, Lane 5: human A549 whole cell lysate, Lane 6: human SHG-44 whole cell lysate, Lane 7: rat brain tissue lysates, Lane 8: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSE antigen affinity purified polyclonal antibody (Catalog # A02930) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSE at approximately 47KD. The expected band size for NSE is at 47KD.
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