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ProductsBack/NSE2 (NSMCE2) Mouse Monoclonal Antibody [Clone ID: OTI3C10]
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human NSMCE2 plasmid (RC207639, hNSMCE2), mouse NSMCE2 plasmid (MR218993, mNSMCE2) using anti-NSMCE2 antibody TA501633 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human NSMCE2 plasmid (RC207639, hNSMCE2), mouse NSMCE2 plasmid (MR218993, mNSMCE2) using anti-NSMCE2 antibody TA501633 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human NSMCE2 plasmid (RC207639, hNSMCE2), mouse NSMCE2 plasmid (MR218993, mNSMCE2) using anti-NSMCE2 antibody TA501633 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

NSE2 (NSMCE2) Mouse Monoclonal Antibody [Clone ID: OTI3C10]

CF501633
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetNSMCE2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    NSE2 (NSMCE2) Mouse Monoclonal Antibody [Clone ID: OTI3C10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3C10
  • Gene ID286053
  • Target name
    NSMCE2
  • Target description
    NSE2 SUMO ligase component of SMC5/6 complex
  • Target synonyms
    C8orf36, MMS21, NSE2, ZMIZ7, E3 SUMO-protein ligase NSE2, E3 SUMO-protein transferase NSE2, NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase, NSMCE2/PVT1 fusion, PVT1/NSMCE2 fusion, methyl methanesulfonate sensitivity gene 21, non-SMC element 2, MMS21 homolog, non-structural maintenance of chromosomes element 2 homolog, zinc finger, MIZ-type containing 7
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96MF7
  • Protein Name
    E3 SUMO-protein ligase NSE2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) NSMCE2 mouse monoclonal antibody, clone OTI3C10 (formerly 3C10)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSMCE2 using anti-NSMCE2 antibody (A06693-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSMCE2 antigen affinity purified polyclonal antibody (Catalog # A06693-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSMCE2 at approximately 34 kDa. The expected band size for NSMCE2 is at 28 kDa.
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