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Western Blot analysis of CoLo cells using NSF Polyclonal Antibody
Western Blot analysis of CoLo cells using NSF Polyclonal Antibody
Western Blot analysis of CoLo cells using NSF Polyclonal Antibody

NSF Antibody

CSB-PA020159
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNSF
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Overview

  • Supplier
    Cusabio
  • Product Name
    NSF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4905
  • Target name
    NSF
  • Target description
    N-ethylmaleimide sensitive factor, vesicle fusing ATPase
  • Target synonyms
    DEE96, SEC18, SKD2, vesicle-fusing ATPase, N-ethylmaleimide-sensitive factor-like protein, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, epididymis secretory sperm binding protein, vesicular-fusion protein NSF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46459
  • Protein Name
    Vesicle-fusing ATPase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: U937 cell lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lymph node lysates; Lane 4: Mouse Cerebellum lysates probed with NSF Polyclonal Antibody, Unconjugated (bs-11255R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of NSF using anti-NSF antibody (A00585-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human 22RV1 whole cell lysates, Lane 8: human HepG2 whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSF antigen affinity purified polyclonal antibody (Catalog # A00585-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSF at approximately 82 kDa. The expected band size for NSF is at 82 kDa.
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