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ProductsBack/NSF Antibody

NSF Antibody

LS-C408266
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNSF
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NSF Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 71kDa/82kDa, while the observed MW by Western blot was 83kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 71kDa/82kDa, while the observed MW by Western blot was 83kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4905
  • Target name
    NSF
  • Target description
    N-ethylmaleimide sensitive factor, vesicle fusing ATPase
  • Target synonyms
    DEE96, SEC18, SKD2, vesicle-fusing ATPase, N-ethylmaleimide-sensitive factor-like protein, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, epididymis secretory sperm binding protein, vesicular-fusion protein NSF
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSF using anti-NSF antibody (A00585-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human 22RV1 whole cell lysates, Lane 8: human HepG2 whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSF antigen affinity purified polyclonal antibody (Catalog # A00585-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSF at approximately 82 kDa. The expected band size for NSF is at 82 kDa.
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