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Western Blot analysis of COLO205 cells using NEEP21 Polyclonal Antibody
Western Blot analysis of COLO205 cells using NEEP21 Polyclonal Antibody
Western Blot analysis of COLO205 cells using NEEP21 Polyclonal Antibody

NSG1 Antibody

CSB-PA003392
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNSG1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NSG1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27065
  • Target name
    NSG1
  • Target description
    neuronal vesicle trafficking associated 1
  • Target synonyms
    brain neuron cytoplasmic protein 1; carboxyterminally EE-tagged neuron-enriched endosomal 21 kDa protein; D4S234; D4S234E; NEEP21; neuron specific gene family member 1; neuronal vesicle trafficking-associated protein 1; neuron-enriched endosomal protein of 21 kDa; neuron-specific protein family member 1; P21
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42857
  • Protein Name
    Neuronal vesicle trafficking-associated protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NSG1 using anti-NSG1 antibody (A12107-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSG1 antigen affinity purified polyclonal antibody (Catalog # A12107-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSG1 at approximately 21 kDa. The expected band size for NSG1 is at 21 kDa.
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