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NSG1 Antibody (Internal)

LS-C368443
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetNSG1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NSG1 Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100 - 1:200), WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27065
  • Target name
    NSG1
  • Target description
    neuronal vesicle trafficking associated 1
  • Target synonyms
    D4S234, D4S234E, NEEP21, P21, neuronal vesicle trafficking-associated protein 1, brain neuron cytoplasmic protein 1, carboxyterminally EE-tagged neuron-enriched endosomal 21 kDa protein, neuron specific gene family member 1, neuron-enriched endosomal protein of 21 kDa, neuron-specific protein family member 1
  • Host
    Rabbit
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSG1 using anti-NSG1 antibody (A12107-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSG1 antigen affinity purified polyclonal antibody (Catalog # A12107-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSG1 at approximately 21 kDa. The expected band size for NSG1 is at 21 kDa.
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