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NSUN6 Antibody

OAAN02176
Aviva Systems Biology
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNSUN6
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    NSUN6 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    WB: 1:500 - 1:2,000. IHC: 1:50 - 1:200. IF: 1:50 - 1:100
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Format
    Liquid
  • Gene ID221078
  • Target name
    NSUN6
  • Target description
    NOP2/Sun RNA methyltransferase 6
  • Target synonyms
    4933414E04Rik, ARL5B-AS1, MRT82, NOPD1, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6, ARL5B antisense RNA 1, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family, member 6, NOP2/Sun RNA methyltransferase family member 6, NOP2/Sun domain family, member 6, nucleolar protein (NOL1/NOP2/sun) and PUA domains 1, putative methyltransferase NSUN6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TEA1
  • Protein Name
    tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NSUN6 using anti-NSUN6 antibody (A13404-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN6 antigen affinity purified polyclonal antibody (A13404-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN6 at approximately 52 kDa. The expected band size for NSUN6 is at 52 kDa.
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