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NTSR1 / NTR Antibody (C-Terminus)

LS-C25750
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNTSR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NTSR1 / NTR Antibody (C-Terminus)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. ELISA: 1:100000. Use 50-100 ng control peptide/well.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:100000), WB (1:1000 - 1:5000) Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. ELISA: 1:100000. Use 50-100 ng control peptide/well.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4923
  • Target name
    NTSR1
  • Target description
    neurotensin receptor 1
  • Target synonyms
    NTR, neurotensin receptor type 1, NT-R-1, NTR1, NTRH, high-affinity levocabastine-insensitive neurotensin receptor, neurotensin receptor 1 (high affinity)
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Neurotensin Receptor 1/NTSR1 using anti-Neurotensin Receptor 1/NTSR1 antibody (A04610-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurotensin Receptor 1/NTSR1 antigen affinity purified polyclonal antibody (Catalog # A04610-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurotensin Receptor 1/NTSR1 at approximately 43 kDa. The expected band size for Neurotensin Receptor 1/NTSR1 is at 43 kDa.
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