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Western Blot analysis of COS7 cells using NUB1 Polyclonal Antibody
Western Blot analysis of COS7 cells using NUB1 Polyclonal Antibody
Western Blot analysis of COS7 cells using NUB1 Polyclonal Antibody

NUB1 Antibody

CSB-PA003491
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetNUB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NUB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51667
  • Target name
    NUB1
  • Target description
    negative regulator of ubiquitin like proteins 1
  • Target synonyms
    BS4; NEDD8 ultimate buster 1; NEDD8 ultimate buster-1; NUB1L; NYREN18; renal carcinoma antigen NY-REN-18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5A7
  • Protein Name
    NEDD8 ultimate buster 1
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUB1 using anti-NUB1 antibody (A06089-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 8: human MCF-7 whole cell lysates, Lane 9: human A549 whole cell lysates, Lane 10: rat liver tissue lysates, Lane 11: rat PC-12 whole cell lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUB1 antigen affinity purified polyclonal antibody (Catalog # A06089-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUB1 at approximately 75 kDa. The expected band size for NUB1 is at 71 kDa.
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