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NUBP1 Antibody

CSB-PA016142XA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNUBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NUBP1 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4682
  • Target name
    NUBP1
  • Target description
    nucleotide binding protein 1
  • Target synonyms
    CIAO5; cytosolic Fe-S cluster assembly factor NUBP1; NBP; NBP 1; NBP1; NBP35; nucleotide binding protein (e.coli MinD like); nucleotide binding protein 1 (E.coli MinD like); nucleotide binding protein 1 (MinD homolog, E. coli)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53384
  • Protein Name
    Cytosolic Fe-S cluster assembly factor NUBP1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUBP1 using anti-NUBP1 antibody (A08538-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human Colo320 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human U20S whole cell lysates, Lane 8: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUBP1 antigen affinity purified polyclonal antibody (Catalog # A08538-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUBP1 at approximately 35 kDa. The expected band size for NUBP1 is at 35 kDa.
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