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Nuclear Mitotic Apparatus Protein (NuMA) Antibody

ORB2318930
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNUMA1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Nuclear Mitotic Apparatus Protein (NuMA) Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Positive Control: Exponentially growing any cultured human cells. Tonsil or lymph node. Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. FC, IF, IHC
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SPM300
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID4926
  • Target name
    NUMA1
  • Target description
    nuclear mitotic apparatus protein 1
  • Target synonyms
    NMP-22, NUMA, nuclear mitotic apparatus protein 1, SP-H antigen, centrophilin stabilizes mitotic spindle in mitotic cells, nuclear matrix protein-22, structural nuclear protein
  • Host
    Mouse
  • Isotype
    IgM
  • Protein IDQ14980
  • Protein Name
    Nuclear mitotic apparatus protein 1
  • Scientific Description
    Nuclear Mitotic Apparatus Protein (NuMA) Antibody
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUMA/NUMA1 using anti-NUMA/NUMA1 antibody (A02018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: human SIHA whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMA/NUMA1 antigen affinity purified polyclonal antibody (Catalog # A02018-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMA/NUMA1 at approximately 270 kDa. The expected band size for NUMA/NUMA1 is at 270 kDa.
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