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Nucleoredoxin (NXN) Polyclonal Antibody

CAU21953
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetNXN
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Overview

  • Supplier
    Biomatik
  • Product Name
    Nucleoredoxin (NXN) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64359
  • Target name
    NXN
  • Target description
    nucleoredoxin
  • Target synonyms
    NRX, RRS2, TRG-4, nucleoredoxin, nucleoredoxin 1
  • Host
    Rabbit
  • Protein IDQ6DKJ4
  • Protein Name
    Nucleoredoxin
  • Scientific Description
    The Nucleoredoxin (NXN) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate All lanes: NXN antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 21, 37 kDa Observed band size: 49 kDa
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Figure 1. Western blot analysis of NXN/NRX using anti-NXN/NRX antibody (A08028-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NXN/NRX antigen affinity purified polyclonal antibody (Catalog # A08028-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NXN/NRX at approximately 48 kDa. The expected band size for NXN/NRX is at 48 kDa.
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