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Western blot All lanes: NUF2 antibody at 5.29microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa
Western blot All lanes: NUF2 antibody at 5.29microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa
Western blot All lanes: NUF2 antibody at 5.29microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa

NUF2 Antibody

CSB-PA871623ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNUF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NUF2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83540
  • Target name
    NUF2
  • Target description
    NUF2 component of NDC80 kinetochore complex
  • Target synonyms
    CDCA1, CT106, NUF2R, kinetochore protein Nuf2, NDC80 kinetochore complex component NUF2, NUF2, NDC80 kinetochore complex component, homolog, cancer/testis antigen 106, cell division cycle associated 1, cell division cycle-associated protein 1, hNuf2, hNuf2R, hsNuf2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZD4
  • Protein Name
    Kinetochore protein Nuf2
  • Scientific Description
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUF2 using anti-NUF2 antibody (A03788-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUF2 antigen affinity purified polyclonal antibody (Catalog # A03788-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUF2 at approximately 54 kDa. The expected band size for NUF2 is at 54 kDa.
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