
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody (GTX110734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
NUF2 antibody
GTX110734
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNUF2
Overview
- SupplierGeneTex
- Product NameNUF2 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.52 mg/ml
- ConjugateUnconjugated
- Gene ID83540
- Target nameNUF2
- Target descriptionNUF2 component of NDC80 kinetochore complex
- Target synonymsCDCA1, CT106, NUF2R, kinetochore protein Nuf2, NDC80 kinetochore complex component NUF2, NUF2, NDC80 kinetochore complex component, homolog, cancer/testis antigen 106, cell division cycle associated 1, cell division cycle-associated protein 1, hNuf2, hNuf2R, hsNuf2
- HostRabbit
- IsotypeIgG
- Protein IDQ9BZD4
- Protein NameKinetochore protein Nuf2
- Scientific DescriptionThis gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161

![NUF2 antibody detects NUF2 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NUF2 stained by NUF2 antibody (GTX110734) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). NUF2 antibody detects NUF2 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NUF2 stained by NUF2 antibody (GTX110734) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX110734/GTX110734_43621_20200205_ICC_IF_w_23060500_987.webp)








![Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT644] (GTX632184) . Western blot analysis was performed using NUF2 antibody [GT644] (GTX632184) diluted at 1:500. EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632184/GTX632184_42044_20160715_IP_w_23061202_330.webp)
![NUF2 antibody [GT312] detects NUF2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NUF2 stained by NUF2 antibody [GT312] (GTX632247) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632247/GTX632247_44706_20230303_IHC-P_23032819_979.webp)