![NUF2 antibody [GT312] detects NUF2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NUF2 stained by NUF2 antibody [GT312] (GTX632247) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min NUF2 antibody [GT312] detects NUF2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NUF2 stained by NUF2 antibody [GT312] (GTX632247) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632247/GTX632247_44706_20230303_IHC-P_23032819_979.webp)
NUF2 antibody [GT312] detects NUF2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NUF2 stained by NUF2 antibody [GT312] (GTX632247) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NUF2 antibody [GT312]
GTX632247
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNUF2
Overview
- SupplierGeneTex
- Product NameNUF2 antibody [GT312]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT312
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID83540
- Target nameNUF2
- Target descriptionNUF2 component of NDC80 kinetochore complex
- Target synonymsCDCA1, CT106, NUF2R, kinetochore protein Nuf2, NDC80 kinetochore complex component NUF2, NUF2, NDC80 kinetochore complex component, homolog, cancer/testis antigen 106, cell division cycle associated 1, cell division cycle-associated protein 1, hNuf2, hNuf2R, hsNuf2
- HostMouse
- IsotypeIgG1
- Protein IDQ9BZD4
- Protein NameKinetochore protein Nuf2
- Scientific DescriptionThis gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody [GT312] (GTX632247) diluted at 1:1000. HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody [GT312] (GTX632247) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632247/GTX632247_42044_20160715_WB_w_23061202_345.webp)
![Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT312] (GTX632247) . Western blot analysis was performed using NUF2 antibody [GT312] (GTX632247) diluted at 1:500. EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent. Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT312] (GTX632247) . Western blot analysis was performed using NUF2 antibody [GT312] (GTX632247) diluted at 1:500. EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632247/GTX632247_42044_20160715_IP_w_23061202_530.webp)








![Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT644] (GTX632184) . Western blot analysis was performed using NUF2 antibody [GT644] (GTX632184) diluted at 1:500. EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632184/GTX632184_42044_20160715_IP_w_23061202_330.webp)