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WB analysis of rat brain lysate using GTX24147 NUMB antibody. Dilution : 0.01microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat brain lysate using GTX24147 NUMB antibody. Dilution : 0.01microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat brain lysate using GTX24147 NUMB antibody. Dilution : 0.01microg/ml Loading : 35microg protein in RIPA buffer

NUMB antibody

GTX24147
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetNUMB
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Overview

  • Supplier
    GeneTex
  • Product Name
    NUMB antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.01-0.03microg/ml. IP: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8650
  • Target name
    NUMB
  • Target description
    NUMB endocytic adaptor protein
  • Target synonyms
    c14_5527; C14orf41; h-Numb; numb homolog; protein numb homolog; S171
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUMB using anti-NUMB antibody (A01206-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87 MG whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMB antigen affinity purified polyclonal antibody (Catalog # A01206-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMB at approximately 72-75 kDa. The expected band size for NUMB is at 42 kDa.
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