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ProductsBack/NUMB Mouse Monoclonal Antibody [Clone ID: OTI4F3]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUMB (Cat# RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB(Cat# TA502168). Positive lysates [LY401229] (100ug) and [LC401229] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUMB (Cat# RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB(Cat# TA502168). Positive lysates [LY401229] (100ug) and [LC401229] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUMB (Cat# RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB(Cat# TA502168). Positive lysates [LY401229] (100ug) and [LC401229] (20ug) can be purchased separately from OriGene.

NUMB Mouse Monoclonal Antibody [Clone ID: OTI4F3]

CF502168
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNUMB
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    NUMB Mouse Monoclonal Antibody [Clone ID: OTI4F3]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4F3
  • Gene ID8650
  • Target name
    NUMB
  • Target description
    NUMB endocytic adaptor protein
  • Target synonyms
    C14orf41, S171, c14_5527, protein numb homolog, h-Numb, numb homolog
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP49757
  • Protein Name
    Protein numb homolog
  • Scientific Description
    Carrier-free (BSA/glycerol-free) NUMB mouse monoclonal antibody, clone OTI4F3 (formerly 4F3)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of NUMB using anti-NUMB antibody (A01206-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87 MG whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMB antigen affinity purified polyclonal antibody (Catalog # A01206-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMB at approximately 72-75 kDa. The expected band size for NUMB is at 42 kDa.
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