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ICC/IF analysis of A549 cells using GTX31351 NUP107 antibody. Working concentration : 10 microg/ml
ICC/IF analysis of A549 cells using GTX31351 NUP107 antibody. Working concentration : 10 microg/ml
ICC/IF analysis of A549 cells using GTX31351 NUP107 antibody. Working concentration : 10 microg/ml

NUP107 antibody

GTX31351
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetNUP107
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Overview

  • Supplier
    GeneTex
  • Product Name
    NUP107 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. ICC/IF: 10 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57122
  • Target name
    NUP107
  • Target description
    nucleoporin 107
  • Target synonyms
    NPHS11; nuclear pore complex protein Nup107; nucleoporin 107kDa; NUP84; ODG6; ODG6; GAMOS7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP57740
  • Protein Name
    Nuclear pore complex protein Nup107
  • Scientific Description
    This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is an essential component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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ICC/IF analysis of A549 cells using GTX31352 NUP107 antibody. Working concentration : 10 microg/ml
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Western Blot Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, PC-3 whole cell lysate All lanes: NUP107 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 107, 104, 80 kDa Observed band size: 107 kDa
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Figure 1. Western blot analysis of NUP107 using anti-NUP107 antibody (A03724-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP107 antigen affinity purified polyclonal antibody (Catalog # A03724-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP107 at approximately 106 kDa. The expected band size for NUP107 is at 106 kDa.
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