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ProductsBack/NUP155 antibody [C1C3]

NUP155 antibody [C1C3]

GTX120945
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetNUP155
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Overview

  • Supplier
    GeneTex
  • Product Name
    NUP155 antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.72 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9631
  • Target name
    NUP155
  • Target description
    nucleoporin 155
  • Target synonyms
    155 kDa nucleoporin; ATFB15; N155; nuclear pore complex protein Nup155; nucleoporin 155kD; nucleoporin 155kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75694
  • Protein Name
    Nuclear pore complex protein Nup155
  • Scientific Description
    Nucleoporins are the main components of the nuclear pore complex (NPC) of eukaryotic cells. They are involved in the bidirectional trafficking of molecules, especially mRNAs and proteins, between the nucleus and the cytoplasm. The protein encoded by this gene does not contain the typical FG repeat sequences found in most vertebrate nucleoporins. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents.
    Read more
  • Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells. Aslanian A et al., 2014 Mar, DNA Repair (Amst)
    Read more
  • The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function. Chow KH et al., 2014 Jan, Mol Biol Cell
    Read more

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Figure 1. Western blot analysis of NUP155 using anti-NUP155 antibody (A06391-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP155 antigen affinity purified polyclonal antibody (Catalog # A06391-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP155 at approximately 140-150 kDa. The expected band size for NUP155 is at 140-150 kDa.
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