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NUP155 Antibody

LS-C409314
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNUP155
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUP155 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 149kDa/155kDa, while the observed MW by Western blot was 155kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 149kDa/155kDa, while the observed MW by Western blot was 155kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9631
  • Target name
    NUP155
  • Target description
    nucleoporin 155
  • Target synonyms
    ATFB15, N155, nuclear pore complex protein Nup155, 155 kDa nucleoporin, nucleoporin 155kD, nucleoporin 155kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUP155 using anti-NUP155 antibody (A06391-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP155 antigen affinity purified polyclonal antibody (Catalog # A06391-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP155 at approximately 140-150 kDa. The expected band size for NUP155 is at 140-150 kDa.
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