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NUP43 Antibody (Biotin)

LS-C397587
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetNUP43
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUP43 Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID348995
  • Target name
    NUP43
  • Target description
    nucleoporin 43
  • Target synonyms
    bA350J20.1, p42, nucleoporin Nup43, nucleoporin 43kDa, nup107-160 subcomplex subunit Nup43
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUP43 using anti-NUP43 antibody (A11950-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP43 antigen affinity purified polyclonal antibody (Catalog # A11950-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP43 at approximately 42 kDa. The expected band size for NUP43 is at 42 kDa.
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Immunohistochemical staining of human adrenal gland, colon, kidney and testis using Anti-NUP43 antibody HPA027126 (A) shows similar protein distribution across tissues to independent antibody HPA027291 (B).
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