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Western blot All lanes: NUP43 antibody at 4microg/ml Lane 1: A431 whole cell lysate Lane 2: HL60 whole cell lysate Lane 3: THP-1 whole cell lysate Lane 4: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 32 kDa Observed band size: 43 kDa
Western blot All lanes: NUP43 antibody at 4microg/ml Lane 1: A431 whole cell lysate Lane 2: HL60 whole cell lysate Lane 3: THP-1 whole cell lysate Lane 4: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 32 kDa Observed band size: 43 kDa
Western blot All lanes: NUP43 antibody at 4microg/ml Lane 1: A431 whole cell lysate Lane 2: HL60 whole cell lysate Lane 3: THP-1 whole cell lysate Lane 4: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 32 kDa Observed band size: 43 kDa

NUP43 Antibody

CSB-PA844045LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNUP43
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Overview

  • Supplier
    Cusabio
  • Product Name
    NUP43 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID348995
  • Target name
    NUP43
  • Target description
    nucleoporin 43
  • Target synonyms
    bA350J20.1, p42, nucleoporin Nup43, nucleoporin 43kDa, nup107-160 subcomplex subunit Nup43
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NFH3
  • Protein Name
    Nucleoporin Nup43
  • Scientific Description
    Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUP43 using anti-NUP43 antibody (A11950-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP43 antigen affinity purified polyclonal antibody (Catalog # A11950-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP43 at approximately 42 kDa. The expected band size for NUP43 is at 42 kDa.
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