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NUP98 Recombinant Antibody

BSM-61197R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNUP98
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Overview

  • Supplier
    Bioss
  • Product Name
    NUP98 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID4928
  • Target name
    NUP98
  • Target description
    nucleoporin 98 and 96 precursor
  • Target synonyms
    ADIR2, NUP196, NUP96, Nup98-96, nuclear pore complex protein Nup98-Nup96, nuclear pore complex protein Nup98, GLFG-repeat containing nucleoporin, NUP98/PHF23 fusion 2 protein, Nup98-Nup96, nucleoporin 96, nucleoporin 98kD, nucleoporin 98kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52948
  • Protein Name
    Nuclear pore complex protein Nup98-Nup96
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate All lanes: NUP98 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 198, 188, 98, 97, 196, 187 kDa Observed band size: 105 kDa
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Figure 1. Western blot analysis of NUP98 using anti-NUP98 antibody (A01301-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP98 antigen affinity purified polyclonal antibody (Catalog # A01301-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP98 at approximately 98,105 kDa. The expected band size for NUP98 is at 198 kDa.
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