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Western Blot analysis of rat stomach 293 cells using NVL Polyclonal Antibody
Western Blot analysis of rat stomach 293 cells using NVL Polyclonal Antibody
Western Blot analysis of rat stomach 293 cells using NVL Polyclonal Antibody

NVL Antibody

CSB-PA004660
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNVL
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Overview

  • Supplier
    Cusabio
  • Product Name
    NVL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4931
  • Target name
    NVL
  • Target description
    nuclear VCP like
  • Target synonyms
    NVL2, nuclear valosin-containing protein-like, NVLp
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15381
  • Protein Name
    Nuclear valosin-containing protein-like
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of NVL using anti-NVL antibody (A05446-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NVL antigen affinity purified polyclonal antibody (Catalog # A05446-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NVL at approximately 95 kDa. The expected band size for NVL is at 95 kDa.
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