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Immunohistochemical analysis of paraffin-embedded D54MG xenograft, using OAS3(GTX118059) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded D54MG xenograft, using OAS3(GTX118059) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded D54MG xenograft, using OAS3(GTX118059) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

OAS3 antibody

GTX118059
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetOAS3
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Overview

  • Supplier
    GeneTex
  • Product Name
    OAS3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.33 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4940
  • Target name
    OAS3
  • Target description
    2'-5'-oligoadenylate synthetase 3
  • Target synonyms
    p100, p100OAS, 2'-5'-oligoadenylate synthase 3, (2-5')oligo(A) synthase 3, (2-5')oligo(A) synthetase 3, 2'-5'-oligoadenylate synthetase 3, 100kDa, 2'-5'oligoadenylate synthetase p100, 2-5A synthase 3, 2-5A synthetase 3, p100 OAS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6K5
  • Protein Name
    2'-5'-oligoadenylate synthase 3
  • Scientific Description
    This gene encodes an enzyme included in the 2, 5 oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2, 5 oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OAS3 using anti-OAS3 antibody (A05032-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAS3 antigen affinity purified polyclonal antibody (Catalog # A05032-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAS3 at approximately 123KD. The expected band size for OAS3 is at 123KD.
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