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Mouse kidney lysates probed with OAT-3 Polyclonal Antibody, Unconjugated (bs-0609R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse kidney lysates probed with OAT-3 Polyclonal Antibody, Unconjugated (bs-0609R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse kidney lysates probed with OAT-3 Polyclonal Antibody, Unconjugated (bs-0609R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

OAT-3 Polyclonal Antibody

BS-0609R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rabbit, Rat
TargetSLC22A8
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Overview

  • Supplier
    Bioss
  • Product Name
    OAT-3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9376
  • Target name
    SLC22A8
  • Target description
    solute carrier family 22 member 8
  • Target synonyms
    OAT3, organic anion transporter 3, hOAT3, organic anion/dicarboxylate exchanger, solute carrier family 22 (organic anion transporter), member 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TCC7
  • Protein Name
    Organic anion transporter 3
  • Reactivity
    Human, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhu L, Dong Y, Na S, et al. Saponins extracted from Dioscorea collettii rhizomes regulate the expression of urate transporters in chronic hyperuricemia rats. Biomed Pharmacother. 2017,93:88-94. doi: 10.1016/j.biopha.2017.06.022
    Read this paper
  • Enoki Y, Watanabe H, Arake R, et al. Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1. Sci Rep. 2016,6:32084. doi: 10.1038/srep32084
    Read this paper
  • Zhou Q, Yu DH, Liu SM, et al. Total saponins from Discorea nipponica makino ameliorate urate excretion in hyperuricemic rats. Pharmacogn Mag. 2015,11(43):567-73. doi: 10.4103/0973-1296.160442
    Read this paper

Datasheet

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Figure 1. Western blot analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT3/SLC22A8 antigen affinity purified polyclonal antibody (Catalog # A04087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT3/SLC22A8 at approximately 62 kDa. The expected band size for OAT3/SLC22A8 is at 60 kDa.
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