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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA240950(SLC22A8 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA240950(SLC22A8 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA240950(SLC22A8 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

SLC22A8 Antibody

CSB-PA240950
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC22A8
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC22A8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9376
  • Target name
    SLC22A8
  • Target description
    solute carrier family 22 member 8
  • Target synonyms
    hOAT3; OAT3; organic anion transporter 3; solute carrier family 22 (organic anion transporter), member 8; solute carrier family 22 member 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TCC7
  • Protein Name
    Solute carrier family 22 member 8
  • Scientific Description
    This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT3/SLC22A8 antigen affinity purified polyclonal antibody (Catalog # A04087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT3/SLC22A8 at approximately 62 kDa. The expected band size for OAT3/SLC22A8 is at 60 kDa.
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