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ProductsBack/Occludin (OCLN) Polyclonal Antibody

Occludin (OCLN) Polyclonal Antibody

CAU24528
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetOcln
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Overview

  • Supplier
    Biomatik
  • Product Name
    Occludin (OCLN) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID18260
  • Target name
    Ocln
  • Target description
    occludin
  • Target synonyms
    Ocl, occludin
  • Host
    Rabbit
  • Protein IDQ61146
  • Protein Name
    Occludin
  • Scientific Description
    The Occludin (OCLN) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Occludin/Ocln using anti-Occludin/Ocln antibody (A01246-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/Ocln antigen affinity purified polyclonal antibody (Catalog # A01246-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/Ocln at approximately 59 kDa. The expected band size for Occludin/Ocln is at 59 kDa.
Product group Antibodies
Boster Bio
Anti-Occludin/Ocln Antibody Picoband(r)A01246-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetOcln
  • SizePrice
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Product group Antibodies
References
Bioss
OCLN Polyclonal AntibodyBS-1495R
ApplicationsFlow Cytometry, Western Blot
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
TargetOcln
  • SizePrice