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ProductsBack/OCLN Polyclonal Antibody
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)

OCLN Polyclonal Antibody

BS-1495R
Bioss
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
TargetOcln
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Overview

  • Supplier
    Bioss
  • Product Name
    OCLN Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID18260
  • Target name
    Ocln
  • Target description
    occludin
  • Target synonyms
    Ocl, occludin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ61146
  • Protein Name
    Occludin
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes. Weber B et al., 2019, PLoS One
    Read this paper
  • Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis. Li N et al., 2019 Aug, Biomed Pharmacother
    Read this paper
  • Bovine alpha-lactalbumin hydrolysates ameliorate obesity-associated endotoxemia and inflammation in high-fat diet-fed mice through modulation of gut microbiota. Li T et al., 2019 Jun 19, Food Funct
    Read this paper
  • Commensal microbiota induces colonic barrier structure and functions that contribute to homeostasis. Hayes CL et al., 2018 Sep 21, Sci Rep
    Read this paper
  • Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response. Zou Y et al., 2016 Dec 16, Sci Rep
    Read this paper
  • Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets. Pan L et al., 2013 Nov 1, Int J Mol Sci
    Read this paper
  • Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets. Zhao Y et al., 2011, Int J Mol Sci
    Read this paper

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Figure 1. Western blot analysis of Occludin/Ocln using anti-Occludin/Ocln antibody (A01246-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/Ocln antigen affinity purified polyclonal antibody (Catalog # A01246-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/Ocln at approximately 59 kDa. The expected band size for Occludin/Ocln is at 59 kDa.
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