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ProductsBack/ODC-1 (Ornithine Decarboxylase-1)(ODC1/487), Biotin conjugate, 0.1mg/mL [26628-22-8]
ODC-1 (Ornithine Decarboxylase-1)(ODC1/487), Biotin conjugate, 0.1mg/mL [26628-22-8]

ODC-1 (Ornithine Decarboxylase-1)(ODC1/487), Biotin conjugate, 0.1mg/mL [26628-22-8]

BNCB0487
Biotium
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
TargetODC1
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Overview

  • Supplier
    Biotium
  • Product Name
    ODC-1 (Ornithine Decarboxylase-1)(ODC1/487), Biotin conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ODC1/487
  • Concentration
    0.1 mg/ml
  • Conjugate
    Biotin
  • Gene ID4953
  • Target name
    ODC1
  • Target description
    ornithine decarboxylase 1
  • Target synonyms
    BABS; NEDBA; NEDBIA; ODC; ornithine decarboxylase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP11926
  • Protein Name
    Ornithine decarboxylase
  • Scientific Description
    Recognizes a 53 kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ODC1 using anti-ODC1 antibody (A03138-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human LNCAP whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODC1 antigen affinity purified polyclonal antibody (Catalog # A03138-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODC1 at approximately 51 kDa. The expected band size for ODC1 is at 51,55 kDa.
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