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ProductsBack/ODC antibody [1G6]
WB analysis of HEK293T cells transfected with ODC plasmid (Right) or empty vector (Left) for 48 hrs using GTX83968 ODC antibody [1G6]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with ODC plasmid (Right) or empty vector (Left) for 48 hrs using GTX83968 ODC antibody [1G6]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with ODC plasmid (Right) or empty vector (Left) for 48 hrs using GTX83968 ODC antibody [1G6]. Loading : 5 ug per lane

ODC antibody [1G6]

GTX83968
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Rat
TargetODC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ODC antibody [1G6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. ICC/IF: 1:100. FCM: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1G6
  • Concentration
    0.57 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4953
  • Target name
    ODC1
  • Target description
    ornithine decarboxylase 1
  • Target synonyms
    BABS, NEDBA, NEDBIA, ODC, ornithine decarboxylase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP11926
  • Protein Name
    Ornithine decarboxylase
  • Scientific Description
    This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq]
  • Reactivity
    Canine, Human, Monkey, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of ODC1 using anti-ODC1 antibody (A03138-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human LNCAP whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODC1 antigen affinity purified polyclonal antibody (Catalog # A03138-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODC1 at approximately 51 kDa. The expected band size for ODC1 is at 51,55 kDa.
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