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ODC1 Antibody (YA2123, Rabbit)

HY-P82378
MedChem Express
TargetODC1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ODC1 Antibody (YA2123, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4953
  • Target name
    ODC1
  • Target description
    ornithine decarboxylase 1
  • Target synonyms
    BABS, NEDBA, NEDBIA, ODC, ornithine decarboxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ODC1 Antibody (YA2123) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ODC1.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ODC1 using anti-ODC1 antibody (A03138-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human LNCAP whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODC1 antigen affinity purified polyclonal antibody (Catalog # A03138-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODC1 at approximately 51 kDa. The expected band size for ODC1 is at 51,55 kDa.
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Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ODC Polyclonal Antibody, Unconjugated (bs-1294R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Western blot All lanes: ODC1 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ODC antibody [N3C3] (GTX101521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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