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OFD1 Polyclonal Antibody

CAC13142
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetOFD1
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Overview

  • Supplier
    Biomatik
  • Product Name
    OFD1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8481
  • Target name
    OFD1
  • Target description
    OFD1 centriole and centriolar satellite protein
  • Target synonyms
    71-7A, CXorf5, JBTS10, RP23, SGBS2, centriole and centriolar satellite protein OFD1, Joubert syndrome type 10, oral-facial-digital syndrome 1 protein, protein 71-7A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75665
  • Protein Name
    Centriole and centriolar satellite protein OFD1
  • Scientific Description
    The OFD1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IF.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA016289LA01HU diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
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Figure 1. Western blot analysis of OFD1 using anti-OFD1 antibody (A02955-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OFD1 antigen affinity purified polyclonal antibody (Catalog # A02955-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OFD1 at approximately 117 kDa. The expected band size for OFD1 is at 117 kDa.
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