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OGA Antibody (YA2041, Rabbit)

HY-P82296
MedChem Express
TargetOGA
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    OGA Antibody (YA2041, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10724
  • Target name
    OGA
  • Target description
    O-GlcNAcase
  • Target synonyms
    MEA5, MGEA5, NCOAT, protein O-GlcNAcase, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase, beta-N-acetylglucosaminidase, beta-N-acetylhexosaminidase, beta-hexosaminidase, bifunctional protein NCOAT, hyaluronidase in meningioma, meningioma expressed antigen 5 (hyaluronidase), meningioma-expressed antigen 5, nuclear cytoplasmic O-GlcNAcase and acetyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    OGA Antibody (YA2041) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2041), targeting OGA, with a predicted molecular weight of 103 kDa (observed band size: 130 kDa). OGA Antibody (YA2041) can be used for WB, IHC-P, IP experiment in human, mouse background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MGEA5/OGA using anti-MGEA5/OGA antibody (A32463). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGEA5/OGA antigen affinity purified polyclonal antibody (Catalog # A32463) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGEA5/OGA at approximately 130 kDa. The expected band size for MGEA5/OGA is at 103 kDa.
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