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Olfactomedin 4 Antibody (YA2288, Rabbit)

HY-P82543
MedChem Express
TargetOLFM4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Olfactomedin 4 Antibody (YA2288, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10562
  • Target name
    OLFM4
  • Target description
    olfactomedin 4
  • Target synonyms
    GC1, GW112, OLM4, OlfD, UNQ362, bA209J19.1, hGC-1, hOLfD, pDP4, olfactomedin-4, G-CSF-stimulated clone 1 protein, antiapoptotic protein GW112, olfactoimedin, tiarin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Olfactomedin 4 Antibody (YA2288) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2288), targeting Olfactomedin 4, with a predicted molecular weight of 57 kDa (observed band size: 54 kDa). Olfactomedin 4 Antibody (YA2288) can be used for WB, IP experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OLMF4 using anti-OLMF4 antibody (A04094-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: human U2OS whole cell lysates Lane 3: human U-87MG whole cell lysates Lane 4: human HL-60 whole cell lysates Lane 5: human K562 whole cell lysates Lane 6: human THP-1 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLMF4 antigen affinity purified polyclonal antibody (Catalog # A04094-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLMF4 at approximately 75KD. The expected band size for OLMF4 is at 57KD.
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