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ProductsBack/OLIG1 antibody [C2C3], C-term

OLIG1 antibody [C2C3], C-term

GTX104823
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetOLIG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    OLIG1 antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID116448
  • Target name
    OLIG1
  • Target description
    oligodendrocyte transcription factor 1
  • Target synonyms
    basic domain, helix-loop-helix protein, class B, 6; BHLHB6; BHLHE21; class B basic helix-loop-helix protein 6; class E basic helix-loop-helix protein 21; oligo1; oligodendrocyte lineage transcription factor 1; oligodendrocyte transcription factor 1; oligodendrocyte-specific bHLH transcription factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TAK6
  • Protein Name
    Oligodendrocyte transcription factor 1
  • Scientific Description
    Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Olig1 using anti-Olig1 antibody (A06108-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Olig1 antigen affinity purified polyclonal antibody (Catalog # A06108-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Olig1 at approximately 28KD. The expected band size for Olig1 is at 28KD.
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