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OLIG1 Polyclonal Antibody

BS-8548R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetOLIG1
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Overview

  • Supplier
    Bioss
  • Product Name
    OLIG1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID116448
  • Target name
    OLIG1
  • Target description
    oligodendrocyte transcription factor 1
  • Target synonyms
    BHLHB6, BHLHE21, oligodendrocyte transcription factor 1, basic domain, helix-loop-helix protein, class B, 6, class B basic helix-loop-helix protein 6, class E basic helix-loop-helix protein 21, oligo1, oligodendrocyte lineage transcription factor 1, oligodendrocyte-specific bHLH transcription factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TAK6
  • Protein Name
    Oligodendrocyte transcription factor 1
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Cong Y, Wang C, Wang J, et al. NT-3 Promotes Oligodendrocyte Proliferation and Nerve Function Recovery After Spinal Cord Injury by Inhibiting Autophagy Pathway. J Surg Res. 2020,247:128-135. doi: 10.1016/j.jss.2019.10.033
    Read this paper

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Figure 1. Western blot analysis of Olig1 using anti-Olig1 antibody (A06108-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Olig1 antigen affinity purified polyclonal antibody (Catalog # A06108-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Olig1 at approximately 28KD. The expected band size for Olig1 is at 28KD.
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