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Western Blot analysis of 293 cells using OMG Polyclonal Antibody
Western Blot analysis of 293 cells using OMG Polyclonal Antibody
Western Blot analysis of 293 cells using OMG Polyclonal Antibody

OMG Antibody

CSB-PA003592
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOMG
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Overview

  • Supplier
    Cusabio
  • Product Name
    OMG Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4974
  • Target name
    OMG
  • Target description
    oligodendrocyte myelin glycoprotein
  • Target synonyms
    OMGP, oligodendrocyte-myelin glycoprotein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23515
  • Protein Name
    Oligodendrocyte-myelin glycoprotein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OMG using anti-OMG antibody (PB9730). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OMG antigen affinity purified polyclonal antibody (Catalog # PB9730) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OMG at approximately 50KD. The expected band size for OMG is at 50KD.
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