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Mouse brain lysates probed with Rabbit Anti-Omgp Polyclonal Antibody, Unconjugated (bs-1200R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Mouse brain lysates probed with Rabbit Anti-Omgp Polyclonal Antibody, Unconjugated (bs-1200R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Mouse brain lysates probed with Rabbit Anti-Omgp Polyclonal Antibody, Unconjugated (bs-1200R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Omgp Polyclonal Antibody

BS-1200R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetOMG
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Overview

  • Supplier
    Bioss
  • Product Name
    Omgp Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4974
  • Target name
    OMG
  • Target description
    oligodendrocyte myelin glycoprotein
  • Target synonyms
    OMGP, oligodendrocyte-myelin glycoprotein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23515
  • Protein Name
    Oligodendrocyte-myelin glycoprotein
  • Reactivity
    Bovine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Feng N, Hao G, Yang F, et al. Transplantation of mesenchymal stem cells promotes the functional recovery of the central nervous system following cerebral ischemia by inhibiting myelin-associated inhibitor expression and neural apoptosis. Exp Ther Med. 2016,11(5):1595-1600.
    Read this paper

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Figure 1. Western blot analysis of OMG using anti-OMG antibody (PB9730). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OMG antigen affinity purified polyclonal antibody (Catalog # PB9730) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OMG at approximately 50KD. The expected band size for OMG is at 50KD.
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