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Oncostatin M Antibody (YA2813, Rabbit)

HY-P83068
MedChem Express
TargetOSM
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Oncostatin M Antibody (YA2813, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5008
  • Target name
    OSM
  • Target description
    oncostatin M
  • Target synonyms
    oncostatin-M
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Oncostatin M Antibody (YA2813) is a non-conjugated IgG antibody, targeting Oncostatin M, with a predicted molecular weight of 28 kDa (observed band size: 22 kDa). Oncostatin M Antibody (YA2813) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (PB9732). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: HUT Whole Cell Lysate, Lane 3: JURKAT Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate, Lane 5: MCF-7 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oncostatin M antigen affinity purified polyclonal antibody (Catalog # PB9732) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oncostatin M at approximately 40 kDa. The expected band size for Oncostatin M is at 28 kDa.
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