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Oncostatin M Recombinant Antibody

BSM-62322R
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetOSM
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Overview

  • Supplier
    Bioss
  • Product Name
    Oncostatin M Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5008
  • Target name
    OSM
  • Target description
    oncostatin M
  • Target synonyms
    oncostatin-M
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13725
  • Protein Name
    Oncostatin-M
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (PB9732). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: HUT Whole Cell Lysate, Lane 3: JURKAT Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate, Lane 5: MCF-7 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oncostatin M antigen affinity purified polyclonal antibody (Catalog # PB9732) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oncostatin M at approximately 40 kDa. The expected band size for Oncostatin M is at 28 kDa.
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