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ProductsBack/OPTN / Optineurin Antibody

OPTN / Optineurin Antibody

LS-C331724
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetOPTN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    OPTN / Optineurin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 59kDa/65kDa, while the observed MW by Western blot was 78kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 59kDa/65kDa, while the observed MW by Western blot was 78kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10133
  • Target name
    OPTN
  • Target description
    optineurin
  • Target synonyms
    ALS12; E3-14.7K-interacting protein; FIP2; FIP-2; GLC1E; HIP7; HIP-7; Huntingtin interacting protein L; huntingtin yeast partner L; huntingtin-interacting protein 7; huntingtin-interacting protein L; HYPL; nemo-related protein; NRP; optic neuropathy-inducing protein; optineurin; TFIIIA-INTP; transcription factor IIIA-interacting protein; transcrption factor IIIA-interacting protein; tumor necrosis factor alpha-inducible cellular protein containing leucine zipper domains
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Optineurin Polyclonal Antibody, Unconjugated (bs-13658R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Figure 1. Western blot analysis of Optineurin using anti-Optineurin antibody (PB9343). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: huamn HepG2 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse heart tissuel lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Optineurin antigen affinity purified polyclonal antibody (Catalog # PB9343) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Optineurin at approximately 75 kDa. The expected band size for Optineurin is at 66 kDa.
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