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WB analysis of human ovary tissue lysate using GTX17287 Orai1 antibody [3F6H5]. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of human ovary tissue lysate using GTX17287 Orai1 antibody [3F6H5]. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of human ovary tissue lysate using GTX17287 Orai1 antibody [3F6H5]. Working concentration : (A) 1 and (B) 2 microg/ml

Orai1 antibody [3F6H5]

GTX17287
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetORAI1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Orai1 antibody [3F6H5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. IHC-P: 2.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3F6H5
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID84876
  • Target name
    ORAI1
  • Target description
    ORAI calcium release-activated calcium modulator 1
  • Target synonyms
    calcium release-activated calcium channel protein 1; calcium release-activated calcium modulator 1; CRACM1; IMD9; ORAT1; protein orai-1; TAM2; TMEM142A; transmembrane protein 142A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96D31
  • Protein Name
    Calcium release-activated calcium channel protein 1
  • Scientific Description
    The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1). [provided by RefSeq, Sep 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ORAI1 using anti-ORAI1 antibody (A00909). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human Jurka whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human K562 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORAI1 antigen affinity purified polyclonal antibody (Catalog # A00909) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORAI1 at approximately 39KD. The expected band size for ORAI1 is at 33KD.
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